Reducing glucoamylase usage for commercial-scale ethanol production from starch using glucoamylase expressing Saccharomyces cerevisiae
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چکیده
منابع مشابه
Production of ethanol from starch by co-immobilized Zymomonas mobilis-glucoamylase in a fluidized-bed reactor.
The production of ethanol from starch was studied in a fluidized-bed reactor (FBR) using co-immobilized Zymomonas mobilis and glucoamylase. The FBR was a glass column of 2.54 cm in diameter and 120 cm in length. The Z. mobilis and glucoamylase were co-immobilized within small uniform beads (1.2-2.5 mm diameter) of kappa-carrageenan. The substrate for ethanol production was a soluble starch. Lig...
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Shwanniomyces occidentalis possess highly efficient alpha amylase activity that enable this yeast to use raw starch as an carbon source. S.occidentalis alpha amylase gene was cloned into the Saccharomyces cerevisiae under the control of different powerful constitutive promoters, ADH1, GPD and TEF. So that the recombinant strains which carries the same gene under the control of different promote...
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Glucoamylase is widely used in the food industry to produce high glucose syrup, and also in fermentation processes for production beer and ethanol. In this work the productivity of the glucoamylase of Aspergillus awamori expressed by the yeast Saccharomyces cerevisiae, produced in submerged fermentation using different starches, was evaluated and characterized physico-chemically. The enzyme pre...
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The feasibility of using pastry waste as resource for glucoamylase (GA) production via solid state fermentation (SSF) was studied. The crude GA extract obtained was used for glucose production from mixed food waste. Our results showed that pastry waste could be used as a sole substrate for GA production. A maximal GA activity of 76.1 ± 6.1 U/mL was obtained at Day 10. The optimal pH and reactio...
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Two commercial strains of Saccharomyces cerevisiae, Saf-Instant (Baker's yeast) and Ethanol red (Mutant) were compared for ethanol production during hot summer season, using molasses diluted up to 6-7 degrees Brix containing 4%-5% sugars. The yeasts were then propagated in fermentation vessels to study the effects of yeast cell count and varying concentrations of Urea, DAP, inoculum size and La...
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ژورنال
عنوان ژورنال: Bioresources and Bioprocessing
سال: 2021
ISSN: 2197-4365
DOI: 10.1186/s40643-021-00375-5